A method for constructing Highway,road, yards or parking it is the replacement of melting and soling. In civil Engineering it is introduced as a bio enzyme named Terrazyme,can be used as soil stabilizer.Day by day the populations are growing with high traffic volume and density that s why highway is required. Sub-base is the actual layer of flexible pavement through which wheel load can be transferred and spread in such a way that it prevents Shear and Unconsolidated deformation.There is a new technology which can improves the different properties of sub soil. It is an experiment to stabilise the soil by using Terrazyme which is a naturally processed bio enzyme and it is ready to use. It is a non-corrosivenon-flammable liquid formation produced by fermentation of vegetable extract that improve geotechnical or engineering properties of soil providing higher soil compaction densities and increasing stability.The main feature of TerraZyme is the remarkable cost saving aspect. TerraZyme saves cost from 15% to 40% in comparison to the conventional system of road construction. Maintenance cost is dramatically reduced up to about 75%. In this study laterite soil with different index properties have been tested for stabilization and strength of stabilized soil is evaluated after the curing period of 0day,7 day,14 days,21days and 28days for different enzyme dosage200ml/3m3,200ml/2.5m3,200ml/2m3,200ml/1.5m3.The obtained results were analyzed and investigated in terms of compaction, Unconfined Compressive Strength (UCS) and California Bearing Ratio (CBR).The changes in engineering properties of the stabilized and unstabilised soils were monitored after curing periods of 0, 7, 15, 21 and 30 days. Changes in compaction properties, UCS and CBR were observed. Based on laboratory data, it has found that laterite treated with terrazyme can give better CBR value. It was also studied that kaloine cannot be treated with Terrazyme.Cohesive soil is mostly preferable for Terrazyme.